Dissociation Reagents
Corning™ Matrigel™ Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
Lonza™ Trypsin-Versene™-Trypsin-EDTA Cell Culture Reagent
Combined reagent used to remove cells from culture vessel surface into suspension.Trypsin-Versene™-Trypsin-EDTA Cell Culture Reagent aids in cell-cell adhesion.
Gibco™ GlutaMAX™ Supplement
An alternative to L-glutamine, with increased stability that improves cell health
Corning™ Matrigel™ GFR Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
Gibco™ Sodium Pyruvate (100 mM)
Growth supplement
Gibco™ ACK Lysing Buffer
Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow
Gibco™ Trypan Blue Solution, 0.4%
Routinely used as a cell stain to assess cell viability using the dye exclusion test
Gibco™ L-Glutamine (200 mM)
Ready-to-use stock solution that acts as a cell culture supplement
Corning™ Matrigel™ hESC-Qualified Matrix
Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.
Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)
A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells
Corning™ Cell-Tak Cell and Tissue Adhesive
Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis.
Corning™ Cell Recovery Solution
Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses
Invitrogen™ Low Density Lipoprotein from Human Plasma, Acetylated, DiI complex (DiI AcLDL)
Acetylated (Ac) apoprotein used to study cell types that express receptors specific for this acetylated version, i.e., endothelial and microglial cells
Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)
A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells
Gibco™ Phytohemagglutinin, M form (PHA-M)
This extract is capable of inducing blastogenesis in vitro in various mammalian mononuclear cells when used at a concentration of 1 to 2% v/v
Gibco™ Distilled Water
High-quality water for use as a solvent in the preparation of cell culture media and laboratory reagents
Invitrogen™ Low Density Lipoprotein from Human Plasma (LDL)
Unlabeled LDL used to study normal cholesterol delivery and internalization
Gibco™ MEM Non-Essential Amino Acids Solution (100X)
Used as a growth supplement for cell culture medium, to increase cell growth and viability
Invitrogen™ Low Density Lipoprotein from Human Plasma, DiI complex (DiI LDL)
Nonacetylated conjugate
Invitrogen™ Zymosan A (S. cerevisiae) BioParticles™, Alexa Fluor™ 594 conjugate
Study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry
Invitrogen™ Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 647 Streptavidin (Alexa Fluor™ 647 EGF complex)
Complexes of the dye-streptavidin molecule and biotinylated EGF
Gibco™ HAT Supplement (50X)
HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells
Gibco™ HT Supplement (100X)
HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.
Corning™ Nu-SerumTM Growth Medium Supplement
Provides a low-serum alternative to newborn calf, fetal bovine, and other sera used for cell culture
Gibco™ KaryoMAX™ Giemsa Stain Solution
For G-banding of chromosomes for cytogenetic analysis
Gibco™ Pluronic™ F-68 Non-ionic Surfactant (100X)
A non-ionic surfactant used to control shear forces in suspension cultures
Invitrogen™ Transferrin From Human Serum, Alexa Fluor™ 555 Conjugate
Labeled LDL complexes useful in studying the delivery of up to two Fe3+ atoms via receptor-mediated endocytosis
Invitrogen™ Transferrin From Human Serum, Alexa Fluor™ 488 Conjugate
Labeled LDL complexes useful in studying the delivery of up to two Fe3+ atoms via receptor-mediated endocytosis
Gibco™ 2-Mercaptoethanol
A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals
Invitrogen™ Low Density Lipoprotein From Human Plasma, Acetylated, Alexa Fluor™ 488 Conjugate (Alexa Fluor™ 488 AcLDL)
Acetylated (Ac) apoprotein used to study cell types that express receptors specific for this acetylated version, i.e., endothelial and microglial cells