Antibiotics, Buffers and Supplements

Gibco™ Penicillin-Streptomycin (10,000 U/mL)

For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria PENICILLIN STREPTOMYCIN SOL

Gibco™ Trypsin-EDTA (0.25%), Phenol red

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas TRYPSIN 0.25% EDTA

GE Healthcare HyClone™ ADCF Antifoam Agent, Irradiated

Prevents and minimizes foaming in bioprocess cell culture systems. GE Healthcare HyClone™ ADCF Antifoam Agent, Irradiated is a gamma radiatopm-sterilized antifoam solution with 3% active simethicone, supplied in a ready-to-use 500mL bag with tube fittings. HyClone 10% Antifoam Solution, ADCF, Irradiated(Q7-2587 Simethicone), 500ml

Corning™ Matrigel™ Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free

Solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. Corning™ Matrigel™ Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free is tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors. Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free (PRF), 10x10mL

Gibco™ Trypsin-EDTA (0.05%), Phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas TRYPSIN .05% EDTA

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression. 5ML Matrigel Basement Membrane Matrix, LDEV-Free

HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached. 100 ML TRYPSIN 0.25% (1X) SOLUTION, WITH 0.1%EDTA Hyclone 100mL Store in Freezer at -20C

Lonza™ Trypsin-Versene™-Trypsin-EDTA Cell Culture Reagent

Combined reagent used to remove cells from culture vessel surface into suspension.Trypsin-Versene™-Trypsin-EDTA Cell Culture Reagent aids in cell-cell adhesion. Trypsin-Versene EDTA Mix 1X 100ml

Gibco™ GlutaMAX™ Supplement

An alternative to L-glutamine, with increased stability that improves cell health 100ML GlutaMAX Supplement

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression. 5ML Matrigel Growth Factor Reduced (GFR) Basement

Gibco™ Trypsin (2.5%), 10X Concentration

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas TRYPSIN, 2.5%, 10X

Gibco™ TrypLE Express Enzyme (1X), No Phenol Red

Unlike porcine trypsin, free of animal-derived components X20 Tryple express kit Store at 2-8C

Gibco™ Sodium Pyruvate (100mM)

Growth supplement 100ML Sodium Pyruvate

Gibco™ ACK Lysing Buffer

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow ACK LYSING BUFFERACK Lysing Buffer is suitable for use in the

Gibco™ Trypsin-EDTA (0.5%), No Phenol Red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas 100ML 0.5% Trypsin-EDTA (10x), no phenol redin plastic container, E-Z hold Store at -20C

Gibco™ Hygromycin B (50mg/mL)

Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus 20 ML HYGROMYCIN B 20ML STORE AT 2-8C

Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test TRYPAN BLUE STAIN

Gibco™ Zeocin™ Zeocin Selection Reagent

Selection antibiotic, formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus ZEOCIN 5G/50ML

Lonza™ Mycoalert™ Mycoplasma Detection Kit

Detect mycoplasma in culture. Mycoalert™ Mycoplasma Detection Kit is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells. MycoAlert Mycoplasma Detection Kit 50 Tests

Gibco™ L-Glutamine (200mM)

Ready-to-use stock solution that acts as a cell culture supplement 20ML L GLUTAMINE, 100X

Gibco™ HEPES (1M)

A zwitterionic organic chemical buffering agent commonly used in cell culture media HEPES (1M) 100ML

Lonza™ BioWhittaker™ Phosphate Buffered Solution (1X)

Serves as a wash solution in a wide variety of laboratory applications.  BioWhittaker™ Phosphate Buffered Solution (1X) Phosphate Buffered Saline is manufactured in accordance with cGMP regulations. PBS-1X, w/o Ca++, Mg++ 1L

Lonza™ BioWhittaker™ HEPES Buffer

Used in open vessels incubated under atmospheric conditions.  BioWhittaker™ HEPES Buffer is manufactured in accordance with cGMP regulations. HEPES Buffer (1M) in normal saline, 100 ml

Gibco™ Bacto™ Yeast Extract

Formulation of culture media for cultivation of heterotrophic microorganisms. Gibco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist. 50KG YEAST EXTRACT

Gibco™ Penicillin-Streptomycin (5,000 U/mL)

For prevention from bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. PENICILLIN STREPTOMYCIN SOL

Lonza™ Mycoalert™ Mycoplasma Assay Control Set

It is recommended to always include the Control Set to ensure that the operator and all of the assay reagents are performing properly. Mycoalert™ Mycoplasma Assay Control Set is designed for use with the MycoAlert™ Mycoplasma Detection Kit. MycoAlert Control Set 10 Tests

Gibco™ TrypLE Express Enzyme (1X), Phenol Red

Animal origin-free, recombinant enzyme X20 Tryple express kit with phenol red store at

Gibco™ B-27™ Supplement (50X), minus vitamin A

Suitable for use in most neuronal cell applications 100ML B-27 SUPPLEMENT W/O VIT A (50X

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells. 5ML MatrigelhESC-Qualified Matrix, LDEV-Free

Lonza™ BioWhittaker™ Phosphate Buffered Saline (10X)

Manufactured in accordance with cGMP regulations and cell culture-tested to assure compatibility PBS-10X, w/o Ca++, Mg++ 1L

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