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IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression
Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.
220.00 € - 286.00 €
Specifications
| Format | Liquid |
|---|---|
| For Use With (Equipment) | StarGate™ System, Mammalian Host Cells |
| Inducing Agent | SV40 large T antigen (e.g. COS1 or COS7) |
| pH | 8 |
| Promoter | Human CMV Immediate-early |
| Product Code | Brand | Description | Protein Tag | Includes | Price | Quantity & Availability | |||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Description | Protein Tag | Includes | Price | Quantity & Availability | |||
|
15653778
|
IBA Lifesciences
5-4400-001 |
pESG-IBA wt1 | Untagged | Ampicillin Resistance cassette and Neomycin resistance gene |
286.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15663778
|
IBA Lifesciences
5-4401-001 |
pESG-IBA wt2 | Untagged | BM40 signal |
286.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15673778
|
IBA Lifesciences
5-4403-001 |
pESG-IBA 3 | Strep-tag for C-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15683778
|
IBA Lifesciences
5-4405-001 |
pESG-IBA 5 | Strep-tag for N-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15693778
|
IBA Lifesciences
5-4423-001 |
pESG-IBA 23 | Strep-tag for C-terminal and GST-tag for N-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15603788
|
IBA Lifesciences
5-4425-001 |
pESG-IBA 25 | GST Tag for N-terminal | Neomycin resistance gene | N/A | ||||
|
15613788
|
IBA Lifesciences
5-4433-001 |
pESG-IBA 33 | His Tag (6x) for C-terminal | Neomycin resistance gene | N/A | ||||
|
15623788
|
IBA Lifesciences
5-4435-001 |
pESG-IBA 35 | His Tag (6x) for N-terminal | Neomycin resistance gene | N/A | ||||
|
15633788
|
IBA Lifesciences
5-4443-001 |
pESG-IBA 43 | His Tag (6x) for N-terminal and Strep-tag for C-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15643788
|
IBA Lifesciences
5-4445-001 |
pESG-IBA 45 | Strep-tag for N-terminal and His Tag (6x) for C-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15653788
|
IBA Lifesciences
5-4462-001 |
pESG-IBA 62 | Strep-tag for C-terminal and FLAG-tag for N-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15663788
|
IBA Lifesciences
5-4463-001 |
pESG-IBA 63 | FLAG-tag for C-terminal | Neomycin resistance gene | N/A | ||||
|
15673788
|
IBA Lifesciences
5-4464-001 |
pESG-IBA 64 | Strep-tag for N-terminal and FLAG-tag for C-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15683788
|
IBA Lifesciences
5-4465-001 |
pESG-IBA 65 | FLAG-tag for N-terminal | Neomycin resistance gene | N/A | ||||
|
15693788
|
IBA Lifesciences
5-4502-001 |
pESG-IBA 102 | Twin-Strep-tag for C-terminal | BM40 signal, Ampicillin Resistance cassette and Neomycin resistance gene |
286.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15603798
|
IBA Lifesciences
5-4503-001 |
pESG-IBA 103 | Twin-Strep-tag for C-terminal | Neomycin resistance gene |
286.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15613798
|
IBA Lifesciences
5-4504-001 |
pESG-IBA 104 | Twin-Strep-tag for N-terminal | BM40 signal, Ampicillin Resistance cassette and Neomycin resistance gene |
286.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15623798
|
IBA Lifesciences
5-4505-001 |
pESG-IBA 105 | Twin-Strep-tag for N-terminal | Neomycin resistance gene |
286.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15633798
|
IBA Lifesciences
5-4523-001 |
pESG-IBA 123 | Twin-Strep-tag for C-terminal and GST-tag for N-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15643798
|
IBA Lifesciences
5-4542-001 |
pESG-IBA 142 | His Tag (6x) for N-terminal and Twin-Strep-tag for C-terminal | BM40 signal and Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15653798
|
IBA Lifesciences
5-4543-001 |
pESG-IBA 143 | His Tag (6x) for N-terminal and Twin-Strep-tag for C-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15663798
|
IBA Lifesciences
5-4545-001 |
pESG-IBA 145 | Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15673798
|
IBA Lifesciences
5-4562-001 |
pESG-IBA 162 | Twin-Strep-tag for C-terminal and FLAG-tag for N-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15683798
|
IBA Lifesciences
5-4564-001 |
pESG-IBA 164 | Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15693798
|
IBA Lifesciences
5-4567-001 |
pESG-IBA 167 | Twin-Strep-tag/FLAG-tag for N-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
|
15603808
|
IBA Lifesciences
5-4568-001 |
pESG-IBA 168 | Twin-Strep-tag/FLAG-tag for C-terminal | Neomycin resistance gene |
266.00 €
5µg |
Please sign in to purchase this item. Need a web account? Register with us today! | |||
Description
pESG-IBA mammalian expression vectorspESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.
- NeoR for generation of stable cells lines.
- Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
- SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
- ColE1 + Amp: for propagation in E. coli
- NeoR: neomycin resistance gene to generate stable cell lines
- BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)
Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Specifications
| Liquid | |
| SV40 large T antigen (e.g. COS1 or COS7) | |
| Human CMV Immediate-early | |
| 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg | |
| 250 ng/μL | |
| Clear |
| StarGate™ System, Mammalian Host Cells | |
| 8 | |
| Acceptor Vector for mammalian expression | |
| 2°C to 8°C for frequent usage, -20°C for long-term storage | |
| 5 μg | |
| Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins |
Safety and Handling
missing translation for 'shelfLife' : 12 months
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