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Macherey-Nagel™ NucleoSpin™ RNA Plant Kit
Isolates total RNA from all kinds of plant material and filamentous fungi without degradation of the RNA. Macherey-Nagel™ NucleoSpin™ RNA Plant Kit is a complete mini spin kit that includes RNA plant columns with collection tubes, filters, all needed buffers and RNase-free rDNase.
Brand: Macherey-Nagel™ 740949.250
This item is not returnable.
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Description
NucleoSpin™ RNA Plant Kit is designed for isolation of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. Typical downstream applications include real-time RT-PCR, gene expression profiling, Northern blotting, primer extension, array technology, and RNase protection assays.
- Two different lysis buffers included—Optimized lysis procedure for typical conditions and for peculiar metabolites
- rDNase included for on-column digestion—Efficient removal of contaminating DNA
- NucleoSpin™ Filters (shredders) included—Efficient sample homogenization and reduction of viscosity
- Parallel purification of DNA possible—By using the NucleoSpin™ RNA/DNA buffer set
Kit Components:
NucleoSpin™ RNA columns with 2mL collection tubes, 2mL and 1.5mL collecting tubes, NucleoSpin™ filters, buffers (lysis, wash, desalting), RNase-free DNase I, reaction buffer for DNase, RNase-free water
- Mini spin column with silica membrane technology
- Yields 3–70μg RNA from 100μg plant material
- Elution volume: 40–60μL
- Fragment size: 200b–20kb
- Binding capacity: 200μg
- Preparation time: 30 min/6 preps
- A260/A280: 1.9–2.1
Specifications
| Mini Spin Column | |
| RNA purification from plant cells and tissue | |
| 250 Preps | |
| 1 Each | |
| 3–70μg |
| Real-time RT-PCR | |
| Lysis buffer RA1 (125mL), Lysis buffer RAP (125mL), Wash buffer RAW2 (80mL), Wash Buffer RA3 concentrate (3 x 25mL), Membrane desalting buffer (125mL), Reaction buffer for rDNase (30mL), RNase-free rDNase (5 size F vials), RNase-free water (60mL), NucleoSpin filters (250), NucleoSpin RNA Plan Columns with collection tubes (250), 2mL Collection tubes (750), 1.5mL Collection tubes (250) | |
| NucleoSpin™ RNA Plant Column | |
| <100mg tissue |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
Supplemental information
- MIXTURE LIST-Contains : ethanol (diluted < 20 %)(denatured with1%IPA/1%MEK, acc.2016/1867/EU); guanidinium thiocyanate; gua