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Macherey-Nagel™ NucleoSpin™ RNA Plant, Mini kit for RNA from plant
Isolates total RNA from all kinds of plant material and filamentous fungi without degradation of the RNA. Macherey-Nagel™ NucleoSpin™ RNA Plant Kit is a complete mini spin kit that includes RNA plant columns with collection tubes, filters, all needed buffers and RNase-free rDNase.
76.90 € - 1655.00 €
Specifications
| Format | Mini Spin Column |
|---|---|
| For Use With (Equipment) | Real-time RT-PCR |
| For Use With (Application) | RNA purification from plant cells and tissue |
| Product Type | NucleoSpin™ RNA Plant Column |
| Quantity | 1 Each |
| Product Code | Brand | No. of Reactions | Includes | Price | Quantity & Availability | ||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | No. of Reactions | Includes | Price | Quantity & Availability | ||||
|
12979894
|
Macherey-Nagel™
740949.10 |
10 Preps | Lysis buffer RA1 (10mL), Lysis buffer RAP (10mL), Wash buffer RAW2 (13mL), Wash Buffer RA3 concentrate (6mL), Membrane desalting buffer (10mL), Reaction buffer for rDNase (7mL), RNase-free rDNase (1 size D vial), RNase-free water (13mL), NucleoSpin filters (10), NucleoSpin RNA Plan Columns with collection tubes (10), 2mL Collection tubes (30), 1.5mL Collection tubes (10) |
76.90 €
10 reactions |
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|
12721021
|
Macherey-Nagel™
740949.50 |
50 Preps | Lysis buffer RA1 (25mL), Lysis buffer RAP (25mL), Wash buffer RAW2 (13mL), Wash Buffer RA3 concentrate (12mL), Membrane desalting buffer (25mL), Reaction buffer for rDNase (7mL), RNase-free rDNase (1 size F vial), RNase-free water (13mL), NucleoSpin filters (50), NucleoSpin RNA Plan Columns with collection tubes (50), 2mL Collection tubes (150), 1.5mL Collection tubes (50) |
380.00 €
50 reactions |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
|
12711021
|
Macherey-Nagel™
740949.250 |
250 Preps | Lysis buffer RA1 (125mL), Lysis buffer RAP (125mL), Wash buffer RAW2 (80mL), Wash Buffer RA3 concentrate (3 x 25mL), Membrane desalting buffer (125mL), Reaction buffer for rDNase (30mL), RNase-free rDNase (5 size F vials), RNase-free water (60mL), NucleoSpin filters (250), NucleoSpin RNA Plan Columns with collection tubes (250), 2mL Collection tubes (750), 1.5mL Collection tubes (250) |
1655.00 €
250 reactions |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Description
NucleoSpin™ RNA Plant Kit is designed for isolation of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. Typical downstream applications include real-time RT-PCR, gene expression profiling, Northern blotting, primer extension, array technology, and RNase protection assays.
- Two different lysis buffers included—Optimized lysis procedure for typical conditions and for peculiar metabolites
- rDNase included for on-column digestion—Efficient removal of contaminating DNA
- NucleoSpin™ Filters (shredders) included—Efficient sample homogenization and reduction of viscosity
- Parallel purification of DNA possible—By using the NucleoSpin™ RNA/DNA buffer set
Kit Components: NucleoSpin™ RNA columns with 2 mL collection tubes, 2 mL and 1.5 mL collecting tubes, NucleoSpin™ filters, buffers (lysis, wash, desalting), RNase-free DNase I, reaction buffer for DNase, RNase-free water
- Mini spin column with silica membrane technology
- Yields 3 to 70μg RNA from 100μg plant material
- Elution volume: 40 to 60μL
- Fragment size: 200b–20kb
- Binding capacity: 200μg
- Preparation time: 30 min./6 preps
- A260/A280: 1.9–2.1
Specifications
| Mini Spin Column | |
| RNA purification from plant cells and tissue | |
| 1 Each | |
| 3–70μg |
| Real-time RT-PCR | |
| NucleoSpin™ RNA Plant Column | |
| <100 mg |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
Supplemental information
- MIXTURE LIST-Contains : ethanol (diluted < 20 %)(denatured with1%IPA/1%MEK, acc.2016/1867/EU); guanidinium thiocyanate; gua
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