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Invitrogen™ Human IL-8 ELISA Kits
Interleukin 8 Recombinant protein SIL8 from Thermo Scientific™ specifically detects Interleukin 8 in Not Applicable samples, and it is validated for Control, ELISA and WB
Brand: Invitrogen™ EH2IL8
This item is not returnable.
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Description
Assay IL-8 (human interleukin-8) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Invitrgen™ Human IL-8 ELISA Kits.
These human IL-8 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-8 (IL-8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-8 antibody pairs and IL-8 standard only. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant human interleukin-8 (IL-8) and has been shown to recognize IL-8 derived from stimulated human umbilical vein endothelial cells
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 25.6 to 1000pg/mL
Sensitivity: < 2pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with with human PF-4, GROα, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-10, IL-12, TNFα, IFNα, IFNγ, or GM-CSF
Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).
Specifications
| 25.6 to 1000pg/mL | |
| Human IL-8 ELISA Kit | |
| Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine | |
| 96 microplate wells | |
| Absorbance at 450nm minus absorbance at 550nm | |
| 1 Plate Kit | |
| Natural and recombinant human interleukin-8 (IL-8) and has been shown to recognize IL-8 derived from stimulated human umbilical vein endothelial cells |
| <2pg/mL | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| No significant cross-reaction with with human PF-4, GROa, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-6, IL-7, IL-10, IL-12, TNFa, IFNa, IFNg, or GM-CSF | |
| Streptavidin-HRP Concentrate, 75µL, TMB Substrate Solution, 13mL, Stop Solution, 13mL, Standard Diluent, 25mL, 30X Wash Buffer, 50mL, Lyophilized Recombinant Human IL-8 Standard, 2 vials, Adhesive Plate Covers, 6 ea, Anti-Human IL-8 Precoated 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 8mL, HRP Dilution Buffer, 14mL | |
| Intra-assay CV <10%; inter-assay CV <10% | |
| 50 μL | |
| 3 hr. |