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Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
Brand: Thermo Scientific™ F170L
535.20 EUR valid until 2024-09-27
Use promo code "21650" to get your promotional price.
Description
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle). The kit also includes Thermo Scientific DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.
Highlights
- Direct PCR—sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps
- Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing
- Specially engineered Phire DNA polymerase—extremely short PCR protocol times
- Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel
Applications
- Genotyping
- Transgene detection
- Knockout analysis
- Sequencing
The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
For optimal results start by accurately calculating your Tm with our Tm calculator.
Specifications
• 2X Phire Tissue Direct PCR Master Mix, 10 x 1.25 mL • Dilution Buffer, 2 x 5 mL • DNARelease Additive, 1.3 mL • 25 μM Control Primer Mix, 40 μL • Nuclease-free water, 10 x 1.25 mL • 50 μg O'GeneRuler Express DNA Ladder Store at -20°C. |
|
Direct PCR | |
Fast | |
Direct PCR Master Mix | |
Genotyping, Hot-start PCR | |
Built-In Hot Start | |
Blunt | |
Tissue |
Medium | |
Phire Hot Start II DNA Polymerase | |
Yes | |
Wet or Dry Ice | |
2X | |
1250 Reactions | |
SuperMix or Master Mix | |
DNA |
For Research Use Only. Not for use in diagnostic procedures.