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Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
Brand: Thermo Scientific™ 34580
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Description
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:
- Sensitivity: pictogram to femtogram sensitivity
- Stability: 8-hr working solution stability; 1-year kit stability at room temperature
- Compatibility: nitrocellulose and PVDF membranes
- Signal duration: 6 to 24 hours
- Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL)
- Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)
SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.
Specifications
| Substrate | |
| Liquid | |
| Nitrocellulose, PVDF | |
| 500 mL | |
| Horseradish Peroxidase | |
| Picogram to high femtogram-level | |
| 60 Mini-Gel Blots, 5000 cm2 of Membrane | |
| Sufficient for 60 mini-gel blots; 5000 cm2 of membrane • SuperSignal West Pico PLUS Luminol/Enhancer, 250 mL • SuperSignal West Pico PLUS Stable Peroxide, 250 mL Store at room temperature. |
| Chemiluminescence | |
| Western Blot | |
| SuperSignal™ | |
| HRP | |
| 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL) | |
| 6 to 24 hr. | |
| SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide | |
| Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.