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Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

Brand: Thermo Scientific™ 34580

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Product Code. 15689364

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Description

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:

Sensitivity: pictogram to femtogram sensitivity
Stability: 8-hr working solution stability; 1-year kit stability at room temperature
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 6 to 24 hours
Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL)
Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

Specifications

Product Type	Substrate
Form	Liquid
Membrane Compatibility	Nitrocellulose, PVDF
Quantity	500 mL
Substrate	Horseradish Peroxidase
Sensitivity	Picogram to high femtogram-level
Sufficient For	60 Mini-Gel Blots, 5000 cm² of Membrane
Content And Storage	Sufficient for 60 mini-gel blots; 5000 cm² of membrane • SuperSignal West Pico PLUS Luminol/Enhancer, 250 mL • SuperSignal West Pico PLUS Stable Peroxide, 250 mL Store at room temperature.

Detection Method	Chemiluminescence
For Use With (Application)	Western Blot
Product Line	SuperSignal™
Conjugate	HRP
Recommended Antibody Concentrations	2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
Signal Duration	6 to 24 hr.
Kit Contents	SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide
Shipping Condition	Room Temperature

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Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

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