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Cytiva StrepTrap™ HP Prepacked Columns
Purify Strep(II)-tagged recombinant proteins by affinity chromatography. Cytiva Life Sciences™ StrepTrap™ HP Prepacked Columns are packed with a robust and stable Sepharose™ based medium which results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also high.
Brand: Cytiva 29048653
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Description
- Prepacked with StrepTactin Sepharose High Performance
- Binding capacity Approx. 6 mg Strep II-tagged protein/ml medium
- Maximum pressure: 3 bar
- Column material: polypropylene
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Generated with 0.5M NaOH
- 1 and 5mL columns
Media
- Ligand: Streptactin
- Matrix: 6% cross-linked agarose
- Average particle size: 34μm
- pH stability working range: >7
1mL columns
- Bed dimensions: 7 x 25mm
- Flow velocity: <4mL/min.
5mL columns
- Bed dimensions: 16 x 25mm
- Flow velocity: <20mL/min.
Specifications
| StrepTactin Sepharose HP prepacked column | |
| 0.27 in. | |
| 1 x 1mL | |
| 25mm | |
| Polypropylene | |
| 3 bar | |
| 34μm | |
| 2° to 8°C | |
| Purification of Strep (II) -tagged recombinant Proteins by affinity chromato graphy |
| 1mL | |
| 7mm | |
| 0.98 in. | |
| <4mL/min. | |
| StrepTactin (∼5mg/mL medium) | |
| 7 | |
| 6% Highly Cross-linked Agarose | |
| Ion Exchange Column |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- StrepTrap HP
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol