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Cytiva StrepTrap™ HP Prepacked Columns
Purify Strep(II)-tagged recombinant proteins by affinity chromatography. Cytiva Life Sciences™ StrepTrap™ HP Prepacked Columns are packed with a robust and stable Sepharose™ based medium which results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also high.
Brand: Cytiva 28-9075-46
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Description
- Prepacked with StrepTactin Sepharose High Performance
- Binding capacity Approx. 6 mg Strep II-tagged protein/ml medium
- Maximum pressure: 3 bar
- Column material: polypropylene
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Generated with 0.5M NaOH
- 1 and 5mL columns
Media
- Ligand: Streptactin
- Matrix: 6% cross-linked agarose
- Average particle size: 34μm
- pH stability working range: >7
1mL columns
- Bed dimensions: 7 x 25mm
- Flow velocity: <4mL/min.
5mL columns
- Bed dimensions: 16 x 25mm
- Flow velocity: <20mL/min.
Specifications
| StrepTactin Sepharose HP prepacked column | |
| 0.27 in. | |
| 5 x 1mL | |
| 25mm | |
| Polypropylene | |
| 3 bar | |
| 34μm | |
| 2° to 8°C | |
| Purification of Strep (II) -tagged recombinant Proteins by affinity chromato graphy |
| 1mL | |
| 7mm | |
| 0.98 in. | |
| <4mL/min. | |
| StrepTactin (∼5mg/mL medium) | |
| 7 | |
| 6% Highly Cross-linked Agarose | |
| Ion Exchange Column |