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IBA Lifesciences StrepMAB-Immo Conjugate
StrepMAB-Immo conjugates are monoclonal antibodies with different fluorescent conjugates to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins
Brand: IBA Lifesciences 2-1564-050
This item is not returnable.
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Description
StrepMAB-Immo fluorescenct conjugates are murine, high-affinity monoclonal antibodies, which are especially suited for detection of Strep-tag™II and Twin-Strep-tag™ fusion proteins in applications like ELISA, FACS, immunohistochemistry, and immunocytochemistry.The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag™II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein).
Proteins containing Strep-tag™II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far, the Twin-Strep-tag™ always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). StrepMAB-Immo is available as conjugate with different fluorescent dyes (DY-488, DY-549, DY-649).
The absorption/emission maxima of the fluorescent dyes are:
• DY-488: 493/518 nm
• DY-549: 550-560/575 nm
• DY-649: 655/676 nm
Please note: For detection of Strep-tag™II or Twin-Strep-tag™ fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin™ is recommended.
Specifications
| 0.10 mL | |
| Solution in PBS; 0.01 % NaN3; 1 mg/ mL BSA | |
| 50 μg | |
| Strep-tag™II and Twin-Strep-tag™ | |
| DY-488 |
| 0.5 mg/mL | |
| Undiluted: 2°C to 8°C, protect from light; Diluted: -25°C to -15°C | |
| ELISA, FACS, immunofluorescence | |
| Conjugate |
For Research Use Only.