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IBA Solutions For Life Science StarGate™ Upstream Fusion Vector pNFUSE-IBA
For upstream positioning of an inserted gene in the final Fusion Donor vector when linking two or more genes cloned in separate Donor Vectors using the StarGate™ transfer cloning system.
Brand: IBA Solutions For Life Science 5-1632-001
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Description
StarGate™ offers a simple cloning procedure for rapid and highly efficient subcloning of an arbitrary gene of interest (GOI) for use in functional expression, purification, detection, and immobilization or separation of recombinant proteins.
- Minimal extra modification of the gene due to short combinatorial sites
- Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
- Genes are cloned in a Donor Vector and then parallel-transfered into Acceptor Vectors that provide different genetic surroundings
- Easily connect two or more genes that are cloned in separate Donor Vectors using Fusion Vectors that are assembled by use of a one-tube reaction
- The GOI to be positioned upstream is transferred into a pNFUSE-IBA derivative, specifying the intergenic region (IR):
- IRES11: Provides internal ribosomal entry site (IRES) from encephalomyocarditis virus for separate cap-independent translation of downstream cistron in mammalian cells
- SD11: IR includes a Shine Dalgarno (SD) sequence for translation of downstream cistron at comparable level to the upstream cistron in bacterial cells
- LINK11: Genes of interest are fused by a sequence encoding the 10 amino acid linker GlySerGlyGlyGlySerGlyGlyGlySer
- LINK12: Genes of interest are fused by a sequence encoding the 15 amino acid linker GlySerGlyGlySerGlyGlyGlyGlySerGlyGlyGlyGlySer
- The GOIs to be positioned downstream are transferred into a pCFUSE-IBA11 vector (identical, irrespective of IR)
Specifications
| Solution, liquid | |
| Resistance: ampicillin | |
| 8 | |
| 20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μL pCFUSE-IBA11 | |
| 250 ng/μL | |
| 5 μg | |
| Upstream positioning, fusing cloned genes of interest |
| Stargate™ system derivatives | |
| IR: Includes a Shine Dalgarno (SD) sequence | |
| Fusion Vector | |
| 2°C to 8°C for frequent usage, -20°C for long-term storage | |
| SD11 | |
| Clear |
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