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IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins.

220.00 €

Specifications

Format	Liquid
For Use With (Equipment)	StarGate™ System, E. Coli BL21(DE3)
Inducing Agent	E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3))
pH	8
Promoter	T7

View More Specs

Products 22

Product Code	Brand	Description	Protein Tag	Price	Quantity & Availability
Product Code	Brand	Description	Protein Tag	Price	Quantity & Availability
15633758 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4200-001	pPSG-IBA wt1	Untagged	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15643758 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4203-001	pPSG-IBA 3	Strep-tag for C-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15653758 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4205-001	pPSG-IBA 5	Strep-tag for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15663758 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4223-001	pPSG-IBA 23	Strep-tag for C-terminal and GST-tag for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15673758 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4225-001	pPSG-IBA 25	GST Tag for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15683758 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4233-001	pPSG-IBA 33	His Tag for C-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15693758 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4235-001	pPSG-IBA 35	His Tag (6x) for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15603768 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4243-001	pPSG-IBA 43	Strep-tag for C-terminal and His Tag (6x) for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15613768 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4245-001	pPSG-IBA 45	Strep-tag for N-terminal and His Tag (6x) for C-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15623768 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4262-001	pPSG-IBA 62	Strep-tag for C-terminal and FLAG-tag for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15633768 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4263-001	pPSG-IBA 63	FLAG-tag for C-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15643768 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4264-001	pPSG-IBA 64	Strep-tag for N-terminal and FLAG-tag for C-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15653768 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4265-001	pPSG-IBA 65	FLAG-tag for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15663768 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4303-001	pPSG-IBA 103	Twin-Strep-tag for C-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15673768 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4305-001	pPSG-IBA 105	Twin-Strep-tag for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15683768 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4323-001	pPSG-IBA 123	Twin-Strep-tag for C-terminal and GST-tag for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15693768 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4343-001	pPSG-IBA 143	Twin-Strep-tag for C-terminal and His Tag (6x) for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15603778 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4345-001	pPSG-IBA 145	Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15613778 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4362-001	pPSG-IBA 162	Twin-Strep-tag for C-terminal and FLAG-tag for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15623778 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4364-001	pPSG-IBA 164	Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15633778 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4367-001	pPSG-IBA 167	Twin-Strep-tag/FLAG-tag for N-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

15643778 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4368-001	pPSG-IBA 168	Twin-Strep-tag/FLAG-tag for C-terminal	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

Description

pPSG-IGA E. coli expression vectors:

pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest.

As the T7 promoter is stronger than the tet promoter, pPSG-IBA vectors are recommended in cases where expression with the tet promoter does not lead to significant yields of the recombinant protein.

High-level expression in E. coli by bacteriophage T7 promoter
High-level transcription by T7 RNA polymerase in BL21 strains
High-level expression of non-toxic proteins
Induction by IPTG
Suitable for in vitro transcription/translation

StarGate System

IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

Minimal extra modification of the gene due to short combinatorial sites
Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
The final expression vector, the Destination Vector, is then placed into the respective host

Specifications

Format	Liquid
Inducing Agent	E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3))
Promoter	T7
Storage Buffer	20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Concentration	250 ng/μL
Vector	Total length: 3469
For Use With (Application)	Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins

For Use With (Equipment)	StarGate™ System, E. Coli BL21(DE3)
pH	8
Product Type	Acceptor Vector for E. coli expression
Content And Storage	2°C to 8°C for frequent usage, -20°C for long-term storage
Quantity	5 μg
Color	Clear

Safety and Handling

missing translation for 'shelfLife' : 12 months

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IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

Specifications

Description

Specifications

Safety and Handling

Documents