Thermo Scientific™ SMART Digest™ Proteinase K Kits

Beschreibung

Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Proteinase K Kits. A significant advance in sample preparation for biopharmaceutical research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.

SMART Digest kits enable you to characterize and quantitate proteins faster, easier, and quicker than ever before. This version of the kit uses proteinase K, which cleaves the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups.

• Significantly increased speed of sample processing
• Simple process
• Easily automate entire protocol for high-throughput processing

• Increased reproducibility over existing protocols
• Higher sensitivity
• Higher data confidence

Typically it takes less than 60 minutes (dependent on sample complexity) to achieve full digestion, whereas an in-solution digest can take up to 24 hours.

Reduction in the number of chemicals used and the immobilized proteinase K design helps mitigate against chemically induced post translational modifications (PTMs) and autolysis. The results are easier to interpret.

When used with the SMART Digest for desalting, Thermo Scientific™ SOLAμ™ SPE solid phase extraction plates provide a fast, easy-to-use, robust, and easily automatable sample preparation solution for analysis of biomolecules.