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Proteintech™ Rat IL-2 ELISA Kit
KE20002 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL2.
Brand: Proteintech™ KE20002-96T
This item is not returnable.
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Description
- The assay recognizes mouse IL2. An antibody specific for IL2 has been pre-coated onto the microwells. The IL2 protein in samples is captured by the coated antibody after incubation.
- Following extensive washing, another antibody of biotinylated specific for IL2 is added to detect the captured IL2 protein.
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
- Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
- Interleukin-2 (IL2) is a protein initially identified as a T cell growth factor. IL2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells.
- For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors
- Nevertheless, it has been shown that IL2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells.
- Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (IL2R).
- IL2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.
Specifications
| NP_446288 | |
| 8.3 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Rat | |
| Rat | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 31.25 to 2000 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 116562 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P17108 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 96TEST Human APOL1 ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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