Thermo Scientific™ POROS™ MabCapture™ A/G Select Resin

Description

POROS™ MabCapture™ A/G Select Resin is a new high-throughput Protein A/G chromatography resin manufactured using our own Protein A/G ligand to enable high-throughput purification performance. This resin allows antibody purification with high dynamic binding performance at low linear flow rates and maintains superior binding capacity at high linear flow rates where other chromatography media products cannot operate.

POROS MabCapture A/G Select Resin provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from a variety of mammalian serum samples. It allows antibody purification with high dynamic binding performance at low flow rates and maintains superior binding capacity at high linear velocities.

• Maintain high dynamic binding capacity at linear flow rates over 700 cm/hr
• Design processes with greater flexibility (shorter bed heights, faster flow rates, or both) due to the improvements made to the antibody binding efficiency
• Obtain the highest dynamic binding capacity for antibodies vs. conventional agarose- or silica-based Protein A or Protein G resins
• Purify polyclonal IgG from nearly any mammalian species
• Sanitize and depyrogenate with standard agents used for other chromatography products
• Recognize major increases to process productivity and reduce the downstream bottleneck

Protein A/G is a chimeric genetically-engineered protein that combines the five IgG binding domains of Protein A and two IgG binding domains of Protein G. Protein A/G is not as pH-dependent as Protein A alone, but otherwise has the additive properties of Protein A and G. Protein A/G binds nearly all isotypes and mammalian species of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples, thereby enabling effective antibody purification.

The development of humanized monoclonal antibodies has continued to fuel the growth of the biotherapeutic market. Addressing the therapeutic demand for monoclonal antibodies has led to major improvements to the cell-based expression systems that are used to manufacture these proteins. Consequently, these improvements have moved the processing bottleneck from upstream cell expression to downstream purification. POROS MabCapture A/G Select chromatography resin offers the capacity, efficiency, and flexibility needed to make improvements to the downstream purification platform, resulting in major increases to process productivity and antibody manufacturing.