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  8. 10659009

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

Brand:  Thermo Scientific™ F548L

249.60 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

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Product Code. 10659009

  • 384.00 € / 500 reactions

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Description

Description

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in reactions when using very short PCR protocols. The unique master mix composition permits usage of short cycling protocols with both low and high complexity DNA templates (15 seconds per kilobase or less). The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.

Features

  • Extreme speed with extension times of 15 s/kb or less
  • Hot start modification allows 'zero-time reactivation'
  • Accurate proofreading DNA polymerase with a fidelity of 25X Taq
  • High yields in reduced cycling time
  • Minimized pipetting steps

Applications

  • Fast PCR
  • High-fidelity PCR
  • Difficult (GC-rich) templates
  • Template generation for sequencing Multiplex PCR
  • Long-range PCR
  • Cloning & mutagenesis
  • Microarray

Notes

  • Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Phusion Flash High-Fidelity PCR Master Mix (5 x 1 mL)

Store at -20°C.
Phusion Flash II DNA Polymerase
High-Fidelity PCR Master Mix
Dry Ice
2X
Built-In Hot Start
Blunt
20 kb or less
High
Fast
500 reactions
Hot-start PCR, High-fidelity PCR
25X
500 Reactions
SuperMix or Master Mix
Colorless
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