Thermo Scientific™ Phire Plant Direct PCR Master Mix

Description

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols, and others.

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria, and yeast. The kit includes a complete set of optimized reagents and detailed protocols, making it an ideal choice for amplification of plant DNA.

Features

• Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
• Available Dilution & Storage protocol allows several PCR reactions from one sample
• Specially engineered Phire Hot Start II DNA Polymerase for extremely short PCR protocol times
• Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Notes

• Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.