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OPTIZYME™ Sau3AI (Bsp143I), Fisher BioReagents™

Brand: Fisher BioReagents BP8030-1

Product Code. 11954792

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Description

5'...^G A T C ...3'
3'... C T A G^...5'

Supplied with: 10X OPTIZYME Buffer Sau3AI
Conditions for 100% Activity:

1X OPTIZYME Buffer Sau3AI: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate, 0.02% Triton X-100 and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 7.4 at 25°C), 10mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with Sau3AI

Compatible Ends: BamHI, BclI, BglII, PsuI

Methylation Effects:

Dam: Completely overlaps - no effect

(No effect on cleavage)

Dcm: Never overlaps - no effect

CpG: May overlap - blocked

3'...CTA Gm5C...5'

(Cleavage is blocked)

EcoKI: Never overlaps - no effect

3'...AC TAG(N)₆ m6ACGA...5'

(No effect on cleavage)

EcoBI: May overlap - no effect

Specifications

Concentration	10 U/μL
Incubator Temperature	37°C
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Sau3AI
Quantity	300 U
Product Type	Sau3AI

Components	10X OPTIZYME™ Buffer Sau3AI
pH	7.4
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
Cut Site	.GATC.
Form	Liquid

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