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OPTIZYME™ PvuI, Fisher BioReagents™
Brand: Fisher BioReagents BP8050-1
This item is not returnable.
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Description
5'...C G A T^C G...3'
3'...G C^T A G C...5'
Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 0 - 20%
- Buffer 2: 20 - 50%
- Buffer 3: 50 - 100%
- Buffer 4: 50 - 100%
- Buffer 5: 100%
- 10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% (v/v) glycerol
- CGm6ATCG
- CGATCG
![TRUSTED_SUSTAINABILITY](/content/dam/fishersci/glyphs/Sustain_Badge.png)
Specifications
10 U/μL | |
37°C | |
>90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with Pvu I | |
Keep container tightly closed | |
CGAT.CG | |
Liquid |
>50% Glycerin, 25 to 50% Water, 1 to 2.5% Sodium Chloride | |
7.4 | |
10mM Tris HCl (pH 7.4 at 25°C), 300mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol | |
300 U | |
PvuI |