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OPTIZYME™ AluI, Fisher BioReagents™

Brand: Fisher BioReagents BP8015-1

Product Code. 11904782

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Description

5'...A G^C T...3'
3'...T C^G A...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1 mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with AluI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: Never overlaps - no effect

EcoKI: Never overlaps - no effect

EcoBI: May overlap - blocked

3'...AC TCGA(N)₅ m6ACGA...5'

(Cleavage is blocked)

Specifications

Concentration	10 U/μL
Incubator Temperature	37°C
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with AluI
Content And Storage	Keep container tightly closed
Cut Site	AG.CT
Form	Liquid

Components	25 to 50% Water, >50% Glycerin
pH	7.4
Storage Buffer	10mM Tris HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
Quantity	600 U
Product Type	Alul

Product Suggestions Safety and Handling

Safety and Handling

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