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Macherey-Nagel™ NucleoSpin™ TriPrep Column Set
For rapid purification of total RNA, DNA, and protein from small and precious samples
Brand: Macherey-Nagel™ 740966.250
This item is not returnable.
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Description
NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein.Superior selectivity - purify your RNA fractionated by size
Isolation of small RNA only (18 to 200 bases)Isolation of small (18 to 200 bases) and large (>200 bases) RNA in two fractionsIsolation of total RNA (small and large in one fraction)
Additional isolation of total protein
Denatured protein ready-to-use for analysis in SDS-PAGE and Western blot.
Excellent RNA recovery - even without phenol/chloroform
Lysis with chaotropic saltSpin column based procedure
Convenient handling - complete equipment provided
NucleoSpin filters - efficient sample homogenisationNucleoSpin protein removal columns - highest purity of small RNArDNase - highly efficient on-column digestion of genomic DNA at RT
- Silica membrane technology
- Format: minispin columns
- Sample size: <10&superscript_7; cultured cells, <30mg human/tissue; <50mg plant tissue, <150μL reaction mixture
- Fragment size, small RNA: 18 to 200 bases
- Fragment size, large RNA: >200 bases
- Typical yield: 10μg small RNA; 95μg large RNA from 10&superscript_7; HeLa cells
- Binding capacity: 200μg
- Elution volume, 30μL to 100μL
- Preparation time: <45min (6 preps human/animal tissue, small and large RNA); <35min (6 preps human/animal tissue, small RNA only)
- Small RNA (18 to 200 bases)
- Large RNA (>200 bases)
- Total RNA (small and large RNA in one fraction), and isolate your total protein fraction For small and large RNA and total RNA purification, and isolation of total protein fraction.
Specifications
| NucleoSpin™ TriPrep Column Set | |
| 2mL Collection Tubes, 1.5mL Collection Tubes, NucleoSpin™ Filters, buffers, RNase-free and rDNase | |
| Spin Column |
| 1 Set | |
| 250rxns | |
| For Rapid Purification of Total RNA, DNA, and Protein From Small and Precious Samples |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- NucleoSpin TriPrep (250)
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P273-Avoid release to the environment.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
Supplemental information
- MIXTURE LIST-Contains : zinc chloride, guanidium thiocyanate, ethanol
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