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Macherey-Nagel™ NucleoSpin™ RNA Columns
Additional silica-membrane mini spin columns for use in isolating RNA of the highest integrity from cells and tissue using the NucleoSpin™ RNA kit.
Brand: Macherey-Nagel™ 740955.50S
This item is not returnable.
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Description
NucleoSpin™ RNA
- rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
- NucleoSpin Filters (shredders) – efficient sample homogenization and reduction of viscosity
- Up to 70 μg ready to use RNA
- Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set
- RNA isolation from cultured cells, tissue (standard protocol):
- RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
- RNA from ≤ 100 μL biological fluids
- RNA clean-up from reaction mixtures
- RNA from samples stored in RNAlater™
- RNA from saliva samples collected with Oragene™ RNA (Genotek)
- Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
Specifications
| Blue band | |
| Mini Spin Column | |
| 40 - 120 μL | |
| Binding capacity: 200 μg | |
| Isolation of RNA from cells and tissue | |
| 50 Columns | |
| 35 min/6 preps |
| rDNase at 4 °C; Other components at room temperature (18 - 25 °C) | |
| Silica Membrane | |
| Cultured Bacterial or Yeast Cells, Human or Animal Tissue | |
| NucleoSpin RNA Kits | |
| Columns only | |
| < 30 mg tissue, 5 x 106 cultured cells |
Safety and Handling
Product Identifier
Supplemental information
- NucleoSpin RNA
Supplemental information
- MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) (denaturedwith 1%IPA/1%MEK, acc.2016/1867/EU); gu
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