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Macherey-Nagel™ NucleoSpin™ RNA Columns
Additional silica-membrane mini spin columns for use in isolating RNA of the highest integrity from cells and tissue using the NucleoSpin™ RNA kit.
Brand: Macherey-Nagel™ 740955.250S
This item is not returnable.
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Description
NucleoSpin™ RNA
- rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
- NucleoSpin Filters (shredders) – efficient sample homogenization and reduction of viscosity
- Up to 70 μg ready to use RNA
- Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set
- RNA isolation from cultured cells, tissue (standard protocol):
- RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
- RNA from ≤ 100 μL biological fluids
- RNA clean-up from reaction mixtures
- RNA from samples stored in RNAlater™
- RNA from saliva samples collected with Oragene™ RNA (Genotek)
- Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
Specifications
| Blue band | |
| Mini Spin Column | |
| 40 - 120 μL | |
| Binding capacity: 200 μg | |
| Isolation of RNA from cells and tissue | |
| 250 Columns | |
| 35 min/6 preps |
| rDNase at 4 °C; Other components at room temperature (18 - 25 °C) | |
| Silica Membrane | |
| Cultured Bacterial or Yeast Cells, Human or Animal Tissue | |
| NucleoSpin RNA Kits | |
| Columns only | |
| < 30 mg tissue, 5 x 106 cultured cells |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- NucleoSpin RNA
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Flammable liquid Category 3
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 1 B
- Skin corrosion/irritation Category 2
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) (denaturedwith 1%IPA/1%MEK, acc.2016/1867/EU); gu
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