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Macherey-Nagel™ Nucleospin™ Plasmid Transfection-grade
A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.
Brand: Macherey-Nagel™ 740490.250
This item is not returnable.
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Description
Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.
- Purifies high-copy plasmids for transfection
- Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
- Rapid purification from E. coli via manual, vacuum or centrifugation processing
- Ultra-fast procedure using mini spin columns simplifies the work-flow
- Downstream applications: transfection of common cell lines (lipofection, electroporation)
- Also suitable for standard molecular biology application, such as cloning and sequencing
Specifications
| <50 EU/ng DNA | |
| Mini Spin Column | |
| Bacterial Culture (E. coli, High-copy Plasmids) | |
| Elution Volume: 30 - 50 μL | |
| Centrifuge, Vacuum manifold (e.g. NucleoVac 96) | |
| NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A | |
| A260/A280: 1.80 - 1.85 | |
| < 15 kbp | |
| 15 - 30 μg |
| Room temperature (18 - 25 °C) | |
| Silica Membrane and Endotoxin-reduction Technology | |
| Binding capacity: 35 μg | |
| Transfection-grade plasmid DNA | |
| Rapid purification of plasmids for transfection | |
| 250 | |
| Up to 5 mL | |
| 14 min/6 preps |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Specific target organ toxicity Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H336-May cause drowsiness or dizziness.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
Supplemental information
- MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt/guanidine hydrochloride/trie