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  7. 15503636

Macherey-Nagel™ Nucleospin™ Plasmid Transfection-grade

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.

Brand:  Macherey-Nagel™ 740490.50


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Product Code. 15503636

  • 120.00 € / 50 reactions

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Description

Description

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

  • Purifies high-copy plasmids for transfection
  • Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
  • Rapid purification from E. coli via manual, vacuum or centrifugation processing
  • Ultra-fast procedure using mini spin columns simplifies the work-flow
  • Downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application, such as cloning and sequencing
Specifications

Specifications

<50 EU/ng DNA
Mini Spin Column
Bacterial Culture (E. coli, High-copy Plasmids)
Elution Volume: 30 - 50 μL
Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A
A260/A280: 1.80 - 1.85
< 15 kbp
15 - 30 μg
Room temperature (18 - 25 °C)
Silica Membrane and Endotoxin-reduction Technology
Binding capacity: 35 μg
Transfection-grade plasmid DNA
Rapid purification of plasmids for transfection
50
Up to 5 mL
14 min/6 preps
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Safety and Handling

Safety and Handling

Signal Word
  • Danger

Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Specific target organ toxicity Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H336-May cause drowsiness or dizziness.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information
  • MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt/guanidine hydrochloride/trie

SDS
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