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Macherey-Nagel™ NucleoSpin™ Plasmid Buffer Set
Used to isolate low-copy plasmids using column chromatography. Macherey-Nagel™ NucleoSpin™ Plasmid Buffer Set is suitable for use only with NuceloSpin™ Plasmid kits. The set includes 75mL of buffers A1 and A2, plus 100mL A3 and RNase A, which is sufficient for 300 additional preps.
Brand: Macherey-Nagel™ 740953
This item is not returnable.
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Description
NucleoSpin™ Plasmid Kit is used for the isolation of low-copy plasmid DNA using a time-saving parallel process. It is suitable for manual or automated processing under vacuum or by centrifugation. It can process up to 5mL of E. coli culture, with a typical yield of 4–6μg of sequencing-grade plasmid DNA per 1mL sample culture.
- NucleoSpin™ Plasmid kits include filter and binding plates/strips, buffers and RNase A
- When a larger amount of low-copy plasmid is required, larger culture volumes can be prepared using additional buffer volumes
- Plasmid Buffer Set provides: 75mL of Resuspension Buffer A1 and Lysis Buffer A2, plus 100mL of Neutralization Buffer A3 and RNase A
- Suitable for use only with NucleoSpin™ Plasmid kits—Sufficient for up to 300 preps
Specifications
| 75mL of Resuspension Buffer A1 and Lysis Buffer A2, plus 100mL of Neutralization Buffer A3 and RNase A | |
| 1 Set | |
| Resuspension Buffer A1, Lysis Buffer A2, Neutralization Buffer A3 |
| NucleoSpin™ Plasmid Kit | |
| Isolation of low-copy plasmid DNA |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
Supplemental information
- MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), guanidine hydrochloride, RNase