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Macherey-Nagel™ NucleoSpin™ miRNA Kit
Isolate small and large RNA in parallel. NucleoSpin™ miRNA Kits work on a wide varitey of tissue and cell culture formats.
Brand: Macherey-Nagel™ 740971.250
This item is not returnable.
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Description
Features
- Parallel isolation of small and large RNA
- RNA purification fractionated by size:
- Isolation of small RNA only (< 200 b),
- Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
- Isolation of total RNA (small and large RNA in one fraction)
- Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
- Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform (patent pending)
- NucleoSpin™ Filters for efficient sample homogenization
- rDNase for efficient on-column removal of genomic DNA
Applications
- Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with phenol / chloroform (e.g., TRIzol™) lysis
- Purification of siRNA and large dsRNA from DICER reactions
- Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization
Specifications
| Mini Spin Column | |
| NucleoSpin™ RNA Columns, NucleoSpin™ miRNA Columns, NucleoSpin™ Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin™ Filters, buffers, RNase-free rDNase | |
| Yes | |
| <45 min./6 Reaction and <35 min./6 Reaction |
| Purification of siRNA and large dsRNA from DICER reactions | |
| 250 | |
| Small RNA: < 200 nt, large RNA: > 200 nt | |
| 10μg small RNA, 95μg large RNA |
Safety and Handling
Hazard Category
- Flammable liquid Category 2
- Respiratory sensitiser Category 1
- Serious eye damage/eye irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 3
- Carcinogenicity Category 2