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Macherey-Nagel™ NucleoSpin™ miRNA Kit
Isolate small and large RNA in parallel. NucleoSpin™ miRNA Kits work on a wide varitey of tissue and cell culture formats.
Brand: Macherey-Nagel™ 740971.10
This item is not returnable.
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Description
Features
- Parallel isolation of small and large RNA
- RNA purification fractionated by size:
- Isolation of small RNA only (< 200 b),
- Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
- Isolation of total RNA (small and large RNA in one fraction)
- Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
- Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform (patent pending)
- NucleoSpin™ Filters for efficient sample homogenization
- rDNase for efficient on-column removal of genomic DNA
Applications
- Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue,
- and in combination with phenol / chloroform (e.g., TRIzol™) lysis
- Purification of siRNA and large dsRNA from DICER reactions
- Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization
Specifications
| Mini Spin Column | |
| NucleoSpin™ RNA Columns, NucleoSpin™ miRNA Columns, NucleoSpin™ Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin™ Filters, buffers, RNase-free rDNase | |
| NucleoSpin™ miRNA Kit | |
| Small RNA: < 200 nt, large RNA: > 200 nt | |
| 10μg small RNA, 95μg large RNA |
| Purification of siRNA and large dsRNA from DICER reactions | |
| 10 | |
| Yes | |
| <45 min./6 Reaction and <35 min./6 Reaction |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 2
- LONG-TERM AQUATIC HAZARD Chronic 3
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 2
- Flammable liquid Category 3
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 1 B
- Specific target organ toxicity Category 3
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H335-May cause respiratory irritation.
- H351-Suspected of causing cancer.
- H411-Toxic to aquatic life with long lasting effects.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P310-Immediately call a POISON CENTER/doctor/.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : 1,4-dioxane, ethanol, guanidinium thiocyanate, 2-mercaptoethanol, rDNase