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Macherey-Nagel Bioanalysis™ NucleoSpin™ 96 Gel and PCR Clean-Up Kit
NucleoSpin™ 96 Gel and PCR Clean-Up Kit provides time saving medium to high throughput PCR clean up.
Brand: Macherey-Nagel Bioanalysis™ 740658.2
This item is not returnable.
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Description
The NucleoSpin™ 96 PCR Clean-up kit allows direct clean-up of PCR reaction mixtures. Within the procedure the addition of chaotropic salt (Buffer NT) allows a reversible adsorption of the PCR products to the silica membrane of the NucleoSpin™ 96 PCR Clean-up Binding Plates. High purity of the PCR-products is achieved by complete removal of primers, primer-dimers, salts, nucleotides, and proteins (e.g., polymerases, BSA) in subsequent washing steps using Buffer NT3. Highly pure PCR products are finally eluted with Elution Buffer NE (5 mM Tris/HCl, pH 8.5) or water (pH 8.5), and can be used directly for further applications.
- Silica membrane technology
- Processing: manual/automated, vacuum/centrifugation
- Sample material: < 10 0μL PCR reaction mixture
- Elution volume: 75 to 150 μL
- Binding capacity: 15 μg
- Preparation time: 45 min/plate
- Fragment size: 65 bp to 10 kb
Specifications
| 18°C to 25°C | |
| PCR clean up | |
| < 100 μL |
| 96-well Plate | |
| 192 Preps |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- Warning
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H302-Harmful if swallowed.
- H412-Harmful to aquatic life with long lasting effects.
Supplemental information
- MIXTURE LIST-Contains : guanidinium thiocyanate
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