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Macherey-Nagel™ NucleoBond™ Xtra Midi Plus EF
Purify endotoxin-free plasmid DNA with high yield. The NucleoBond™ Xtra Midi Plus EF Kit represents the new generation of anion exchanger allowing high yield, ultra-fast. The Plus kit contains NucleoBond™ Finalizers to speed up DNA precipitation.
Brand: Macherey-Nagel™ 740422.50
This item is not returnable.
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Description
Features
- Endotoxin-free
- Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells
- Patented endotoxin removal by additional washing step – no extra ice incubation necessary
- Optimized column design – Midi prep in 45 min.
- Column filter included – high filter flow-rates, parallel lysate clearing and loading onto the column
- Improved silica material – typical yields of 400μg (Midi)
- NucleoBond™ Xtra EF kits contain NucleoBond™ Finalizers to speed up DNA precipitation
Applications
- High-copy plasmids from E. coli
- Low-copy plasmids from E. coli (increased buffer volumes required)
Specifications
| Yes | |
| Anion Exchange Chromatography Technology | |
| 50 | |
| Yes | |
| <300kbp |
| Midi Gravity-flow Column | |
| Nucleo Bond Xtra Midi Plus EF | |
| Nucleo Bond Xtra Midi Plus EF | |
| < 200, < 400μL | |
| 400μg |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Flammable liquid Category 3
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
Hazard Statement
- H226-Flammable liquid and vapour.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
Supplemental information
- MIXTURE LIST-Contains : 2-propanol/ethanol (diluted < 20 %)/sodium hydroxide solution (diluted < 2 %), dodecyl sulfate, sodi