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Macherey-Nagel™ Binding Buffer NTC
Cleans up single-stranded DNA. Macherey-Nagel™ Binding Buffer NTC is designed to obtain higher yields for small fragments using the NucleoSpin™ Gel and PCR Clean-Up Kit. It is used to purify short, single-stranded DNA and to extract small, single-stranded RNA from agarose gels.
Brand: Macherey-Nagel™ 740654.100
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Description
NucleoSpin™ Gel and PCR Clean-Up is a two-in-one kit with optimized recovery and elution volume for both PCR purification and gel extraction. The protocol ensures complete removal of contaminations for use in downstream applications, such as cloning, sequencing, PCR and restriction analysis.
Binding Buffer NTC is a supplemental buffer that obtains higher yields for small fragments compared to Buffer NTI, which is included in the kit. Generally, 200μL Buffer NTC is mixed with 100μL sample or 100mg agarose gel <2%. (Use 400μL NTC for 100mg agarose gel >2%.)
Applications:
- Clean-up/purification of short, single-stranded DNA
- Extract small, single-stranded RNA from agarose gels
- Achieve full recovery of fragments <50bp, even primer oligonucleotides
Specifications
| NucleoSpin™ Gel and PCR Clean-Up Kit | |
| 100 mL | |
| For clean-up of single-stranded DNA and for Extraction of single-stranded RNA from agarose gel | |
| Binding Buffer NTC |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- Warning
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H302-Harmful if swallowed.
- H312-Harmful in contact with skin.
- H332-Harmful if inhaled.
- H412-Harmful to aquatic life with long lasting effects.
- EUH031-Contact with acids liberates toxic gas.
Supplemental information
- MIXTURE LIST-Contains : potassium thiocyanate
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