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Invitrogen™ Ni-NTA Agarose
Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
Brand: Invitrogen™ R90115
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Includes: The Ni-NTA resin is pre-charged and able to bind up to 50mg of recombinant protein per 1mL of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+
Description
- Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
- Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine
- One-step purification can be performed under both native and denaturing conditions
- Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications
- Guaranteed stable for 6 months when properly stored at 4°C
Specifications
| The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored. | |
| Agarose | |
| Suspension |
| Ni-NTA | |
| 25 mL | |
| Affinity Column |
For Research Use Only. Not for use in diagnostic procedures.