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Invitrogen™ IFN gamma Mouse ELISA Kit

Quantifies natural and recombinant mouse IFN γ in serum, plasma, buffered solution, or cell culture medium

668.00 € - 2145.00 €

Specifications

Accession Number P01580
Assay Range 47-300 pg/mL
Assay Sensitivity <2 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

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Description

Description

The Mouse Interferon Gamma (Ms IFNγ) ELISA quantitates Ms IFNγ in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IFNγ. Principle of the method The Mouse IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interferon-gamma (IFN γ) is a lymphokine produced by activated T and NK cells.

Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

P01580
<2 pg/mL
ELISA Kit
Mouse
Colorimetric Microplate Reader
IFN-g,Ifg
6.5%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
1 hr. 20 min.
47-300 pg/mL
Biotin
Plasma, Serum, Supernatant
Colorimetric Detection
15978
4 hr.
3.2%
HRP
Plasma, 25 μL; Serum, 25 μL; Supernatant, 25 μL
Mouse
4 hr.
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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