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MP Biomedicals™ Gnome™ DNA Isolation Kit
Gnome™ DNA Isolation Kit offers a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types.
Brand: MP Biomedicals™ 112010600
This item is not returnable.
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Description
- No organic extractions are required, and up to 100 μg of DNA can be isolated per prep.
- The GNOME™ kit utilizes RNase Mixx to eliminate RNA immediately after lysis, and Protease Mixx to rapidly degrade cellular proteins. This is followed by a proprietary ''salting-out'' technique which precludes the need for phenol, chloroform, or other organic extractions.
- The DNA can either be spooled, removed with forceps, or centrifuged and dissolved in TE.
- The GNOME™ DNA Isolation kit is used to quickly and efficiently isolate high molecular weight genomic DNA from cells and tissues of any type, including bacteria, yeast, plant, and animal cells.
- Each preparation with the GNOME™ kit yields up to 100 μg of genomic DNA.
- The DNA isolated by the GNOME™ procedure is suitable for restriction enzyme digestion or PCR amplification in as little as 1 hour after cell lysis.
Specifications
| Kit | |
| Cell Lysis/Denaturing Solution, RNase Mixx, Protease Mixx, Salt Out Mixture, Cell Suspension Solution | |
| Cell Lysis/Denaturing Solution pH range: 6.2 to 6.9, Salt-out Mixture pH range: 6.0 to 6.8, Cell Suspension Solution pH range: 8.0 to 8.2 | |
| 20 to 50 mg wet weight bacterial cell pellet, Up to 250 mg plant tissue, 2 to 5 x 107 nucleated white blood cells, 50 to 250 mg mammalian tissue, ∼1 to 5 x 108 yeast cell pellet |
| Blood, Tissues, Yeast, Bacteria, Plant | |
| 100 Preps | |
| Gnome™ DNA Isolation Kit | |
| 100 μg |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- gnome DNA isolation kit
Signal Word
- Warning
Hazard Category
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
Supplemental information
- MIXTURE LIST-Contains : sodium dodecyl sulphate
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.