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Invitrogen™ Mouse TNF alpha ELISA Kits
Measure levels of mouse tumor necrosis factor alpha (TNFa) in mouse serum and cell culture supernatant samples using 96-well plates.
Brand: Invitrogen™ EMTNFA5
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Description
Assay TNFα (mouse tumor necrosis factor alpha) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse TNF alpha ELISA Kits.
These mouse TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant mouse TNFα (mouse tumor necrosis factor alpha)
Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 50 to 2450pg/mL
Sensitivity: < 9pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, serum
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNα or human TNFα. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFα in culture supernatants
Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).
Specifications
| 50 to 2450pg/mL | |
| Mouse TNF alpha ELISA Kit | |
| Cell Culture Supernatant, Serum | |
| 5 x 96 microplate wells | |
| Absorbance at 450nm minus absorbance at 550nm | |
| 5 Plate Kit | |
| Natural and recombinant mouse TNFa (mouse tumor necrosis factor alpha) |
| <9pg/mL | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNa or human TNFa. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFa in culture supernatants | |
| Streptavidin-HRP Concentrate, 250µL, TMB Substrate Solution, 5 x 13mL, Stop Solution, 55mL, Standard Diluent, 55mL, Lyophilized Recombinant Mouse TNF alpha Standard, 5 vials, HRP Dilution Buffer, 70mL, Biotinylated Antibody Reagent, 30mL, Anti-Mouse TNF alpha Precoated 96-well Strip Plate, 5 ea, Adhesive Plate Covers, 30 ea, 30X Wash Buffer, 200mL | |
| Intra-assay CV <10%; inter-assay CV <10% | |
| 50 μL | |
| 3 hr. |