Invitrogen™ CellROX™ Deep Red Reagent, for oxidative stress detection

Description

A novel fluorogenic probe for measuring cellular oxidative stress in both live and fixed cell imaging, with absorption/emission maxima at ∽644/665 nm. The cell-permeant dye is non-fluorescent while in reduced state, and exhibits bright fluorescence upon oxidation by reactive oxygen species (ROS).

• Simple protocol that can be used with other live cell dyes and GFP
• Compatible with both live cell fluorescence-imaging and formaldehyde-based fixation methods
• Volume: 250µL (liquid)
• Excitation Class: Visible

Substrate Specifics

• CellROXDeep Red reagent is novel cell-permeant dye with absorption/emission maxima of approx. 644/665nm
• CellROXDeep Red reagent is non-fluorescent while in reduced state and becomes fluorescent upon oxidation by reactive oxygen species with emission maxima approx. 665nm that is measurable by fluorescent imaging, high content imaging, fluorescent plate readers, or flow cytometry

• Oxidative stress results from an imbalance between production of reactive oxygen species (ROS) and ability of cells to scavenge them
• ROS play important role in progression of several diseases including inflammation, atherosclerosis, aging, and age-related degenerative disorders
• CellROXDeep Red Reagent detects oxidative stress in cells by reacting with ROS to become brightly fluorescent

• Protocol to use this reagent is simple and bright deep red fluorescence signal is compatible with other live cell dyes and GFP, making it useful in multiplex fluorescence assays to measure variety of cellular phenomena, including parameters related to cytotoxicity and cell death
• Unlike many other ROS sensors, such as H2DCFDA, the signal from CellROXDeep Red is retained after formaldehyde fixation for flexibility with assay and improved workflows compared to those based on classic dyes for ROS detection

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, High-Content Screening (HCS), Nitro-Oxidative Stress, Reactive Oxygen Species

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