Invitrogen™ Calcium Green™-1 dextran, Potassium Salt, 3000 MW, Anionic

Description

• Uses in many calcium signaling investigations, including measuring intracellular Ca²⁺, following Ca²⁺ influx and release, and multiphoton excitation imaging of Ca²⁺ in living tissues
• Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated indicators using patch pipet, microinjection, or Influx™ pinocyte-loading reagent
• Fluorescence signal from these cells is measured using fluorescence microscopy
• Dextran forms of calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms
• Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength
• These probes are excited by visible light, and because the energy required for excitation is low, the potential for cellular photodamage is reduced
• Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of the spectrum where cellular autofluorescence and scattering backgrounds are often less of a problem

• Label (Ex/Em): Calcium Green-1 (506/531nm)
• Fluorescence intensity increase upon binding Ca²⁺: approximately 14 fold

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Endocytosis, Exocytosis and Phagocytosis, Ionic Homeostasis and Signaling

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