Invitrogen™ Amplex™ Red Cholesterol Assay Kit

Description

Designed for minimal autofluorescence interference, the Amplex Red Cholesterol Assay Kit provides a sensitive, rapid, and simple fluorometric method for detecting very low concentrations of cholesterol using a fluorescence microplate reader or fluorometer.

• Detects as little as 200nm (80ng/mL) cholesterol
• Helps accurately measure cholesterol content of 0.01μL of human serum
• Detects both free cholesterol and cholesteryl esters
• Allows for multiple time point measurements
• Designed for minimal autofluorescence interference
• Custom assay design and packaging are also available

Cholesterol Measurement is Simple and Fast

• Quick and easy assay, requiring only 30-minute incubation after addition of reagents with no separation steps required
• Assay volumne of 100μL is designed for 96-well plates
• Fluorescence can be measured with microplate reader or fluorometer
• Since the assay is continuous, once the Amplex Red reagent is added, multiple time-point measurements can be taken
• Complete kit with all necessary reagents except deionized water

Helps Accurately Detect Very Low Cholesterol Concentrations

• Utilizes enzyme-coupled reactions to produce highly fluorescent resorufin for quantitation
• Resorufin is produced by the reaction of the Amplex Red reagent with H₂O₂ produced from the cholesterol oxidase-catalyzed oxidation of cholesterol
• Large proportion of cholesterol in blood is in the form of cholesteryl esters and hence cholesterol esterase is used to produce free cholesterol from cholesterol esters
• Performing the assay in the presence and absence of cholesterol esterase potentially allows determination of the fractions of cholesterol in each form

Use Amplex Red Assays for a Broad Range of Investigations
Wide variety of validated Invitrogen Amplex Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex UltraRed, a second-generation reagent providing greater sensitivity and brighter fluorescence and the Amplex Red/UltraRed stop reagent. The Amplex Red/UltraRed stop reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least 3 hours.

Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis

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