missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Merck Immobilon Western HRP Chemiluminescence Substrates
Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns
Brand: Merck WBLUF0500
This item is not returnable.
View return policy
Description
Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.
- Consistent results with less pipetting error
The stabilizers added to Luminata substrates:
- Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
- Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
- Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation
Three different sensitivities for a range of detection needs:
- Immobilon Classico premixed reagent for everyday detection needs
- Immobilon Crescendo premixed reagent for detection in the picogram range
- Immobilon Forte premixed reagent for detection in the femtogram range
Specifications
| HRP Substrate | |
| 500 mL | |
| 18°C to 24°C |
| Dot/Slot Blot | |
| ∼400 fg |