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Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit
The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns.
Brand: Invitrogen™ K210010
Includes: Resuspension Buffer (R3)
RNase A (20 mg/mL)
Lysis Buffer (L7)
Precipitation Buffer (N4)
Wash Buffer (W9)
Wash Buffer (W10)
TE Buffer (TE)
Spin Columns
Wash and Recovery Tubes
Description
The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns. Features of the PureLink™ Quick Plasmid Miniprep Kit:
- Fast—ready-to-use plasmid DNA typically in about 30 min
- Flexible—formats work with vacuum and centrifugation platforms
Reliable and cost-effective isolation of plasmid DNA
The PureLink™ Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30–45 min, using the PureLink™ spin columns. These columns use a unique silica membrane to achieve high yields of up to 40 μg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA. Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer. The eluted DNA is suitable for all routine downstream applications.
Compatible with flexible formats
The PureLink™ Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA. Typically, DNA isolated using the PureLink™ Quick Plasmid Miniprep Kit has an A260/A280 of >1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.
Isolated DNA can be used for a variety of downstream applications
Plasmid DNA purified using PureLink™ Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR.
Order Info
Shipping Condition: Room Temperature
Specifications
| 20 mg/mL | |
| • Resuspension buffer • RNase A (20 mg/mL) • Lysis buffer • Precipitation buffer • Wash buffers • TE buffer • Spin columns in wash tubes • Elution tubes |
|
| Silica Spin Column | |
| Plasmid DNA | |
| Not High-throughput Compatible (Manual) | |
| < 100 μg (Small-Scale) Plasmid DNA | |
| Quick Plasmid MiniPrep Kit | |
| Room Temperature | |
| 30 to 45 min. |
| Spin Column | |
| Spin Column | |
| Bacterial Culture | |
| Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation | |
| 50 Preps | |
| PureLink™ | |
| 50 Preps | |
| Plasmid DNA |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- PureLink Quick Plasmid Miniprep Kit
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P264-Wash thoroughly after handling.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
Supplemental information
- MIXTURE LIST-Contains : Sodium azide, Sodiumhydroxide, Guanidine hydrochloride, Guanidine hydrochloride
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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