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Invitrogen™ PureLink™ Genomic DNA Mini Kit

Brand: Invitrogen™ K182000

42.38 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

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Product Code. 10593245

56.50 € / Each

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Description

The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink™ Genomic DNA Mini Kit include:

• Kit flexibility—one kit works with a variety of sample types and sizes
• Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
• Improved design—optimized spin-column design and buffer formulation for better yield and purity

Multiple sample sources, one kit
The PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Optimized components and protocols
The PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use. Columns are newly configured to bind and release DNA more efficiently. Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

Higher yields and purity
With PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A₂₆₀/A₂₈₀ measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells. The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀, indicating no detectable contamination from carryover of protein or guanidine.

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage	• 2.0 mL PureLink™ Genomic Lysis/Binding Buffer • 1.8 mL PureLink™ Genomic Digestion Buffer • 2.0 mL PureLink™ Genomic Wash Buffer 1 • 1.5 mL PureLink™ Genomic Wash Buffer 2 • 2.0 mL PureLink™ Genomic Elution Buffer • 0.4 mL RNase A (20 mg/mL) • 0.2 mL Proteinase K (20 mg/mL) • 10 PureLink™ Spin Columns with Collection Tubes • 20 PureLink™ Collecion Tubes (2.0 mL) Store all components at room temperature.
Scale	Mini
For Use With (Application)	Genotyping, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing, PCR
Prep Scale	100 μL to 1 mL Blood Samples (Medium-Scale)
Product Type	Genomic DNA Mini Kit
Shipping Condition	Room Temperature

Isolation Technology	Silica Spin Column
Sample Type	Bacteria, Blood, Cells, Tissue
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Product Line	PureLink™
Quantity	10 Preps
Target	Genomic DNA

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

Genomic DNA Mini Kit

Signal Word

Danger

Hazard Category

Acute toxicity Category 4
Serious eye damage/eye irritation Category 2
Respiratory sensitiser Category 1
Skin corrosion/irritation Category 2

Hazard Statement

H302-Harmful if swallowed.
H315-Causes skin irritation.
H319-Causes serious eye irritation.
H332-Harmful if inhaled.
H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P264-Wash hands thoroughly after handling.
P270-Do not eat, drink or smoke when using this product.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P284-¢In case of inadequate ventilation! wear respiratory protection.
P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P330-Rinse mouth.
P332+P313-If skin irritation occurs: Get medical advice/attention.
P337+P313-If eye irritation persists: Get medical advice/attention.
P362+P364-Take off contaminated clothing and wash it before reuse.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : Guanidine hydrochloride

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Invitrogen™ PureLink™ Genomic DNA Mini Kit

Description

Order Info

Specifications

Product Suggestions

Safety and Handling

Documents

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