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Invitrogen™ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
Brand: Invitrogen™ 10342046
Includes: Taq DNA Polymerase (5U/μL); 10X PCR buffer (200mM Tris-HCl pH 8.4, 500mM KCl); Magnesium Chloride (50mM)
Description
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5'→3' DNA polymerase activity and a 5'→3' exonuclease activity.
With our Taq DNA Polymerase, you get:
- Your choice of recombinant or native enzyme
- Amplification of PCR products up to 5 kb in size
- An enzyme that is licensed and qualified for PCR
Applications
Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing.
Source
Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min. at 74°C.
For superior PCR performance, we recommend DreamTaq DNA Polymerase.
Order Info
Shipping Condition: Approved for shipment on wet or dry ice
Specifications
| • Taq DNA Polymerase (5 U/μL), 3 x 100 μL • 10X PCR buffer, 7.5 mL • 50 mM MgCl2, 3 mL Store at -10°C to -30°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly. |
|
| Low | |
| Taq DNA Polymerase | |
| 5' - 3' | |
| 3 x 500 Units | |
| Standard PCR | |
| 1X | |
| 1500 Reactions | |
| Separate Components | |
| DNA |
| Stand-alone enzyme | |
| qPCR, Standard PCR | |
| Standard | |
| DNA Polymerase | |
| Dry Ice | |
| 5 U/μL | |
| No | |
| 3'-A | |
| 5 kb or less |
For Research Use Only. Not for use in diagnostic procedures.