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Invitrogen™ AccuPrime™ Pfx SuperMix
AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.
Brand: Invitrogen™ 12344040
501.00 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Includes: AccuPrime Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each (4 x 1.125mL) are provided. AccuPrime Pfx SuperMix Components: 22U/mL Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66mM Tris-SO4 (pH 8.4), 30.8mM (NH4)2SO4, 11mM KCl, 1.1mM MgSO4, 330μM dNTPs, AccuPrime proteins, stabilizers.
Requires : Template and primers
This item is not returnable. View return policy
Description
Invitrogen AccuPrime Pfx SuperMix provides reagents for high-fidelity, high-specificity amplification of DNA fragments. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. This ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs is designed for efficient PCR amplification.
Features
- >26-times higher fidelity than with Taq DNA polymerase
- Reduced set-up time
- Produces products with blunt ends
Applications
- High-fidelity PCR
- Amplification of fragments up to 15 kb
- Cloning & mutagenesis
Notes
- Thermostable AccuPrime Pfx SuperMix proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.
Order Info
Approved for shipment on Wet or Dry Ice
Specifications
| AccuPrime Pfx SuperMix (4 × 1.125 mL) Store at –20°C in a non-frost-free freezer. |
|
| Low | |
| Standard | |
| Hot-start PCR, High-fidelity PCR | |
| 26X | |
| 200 Reactions | |
| SuperMix or Master Mix |
| Primer-probe | |
| AccuPrime Pfx DNA Polymerase | |
| Wet or Dry Ice | |
| 1.1X | |
| Built-In Hot Start | |
| Blunt | |
| 15 kb or less |
For Research Use Only. Not for use in diagnostic procedures.