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Cytiva Single Cell GenomiPhi™ DNA Amplification Kit
Amplify whole genome DNA from as little as a single cell. Cytiva Lifescience™ illustra™ Single Cell GenomiPhi™ DNA Amplification Kit combines unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.
510.00 € - 1833.00 €
Specifications
Content And Storage | Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C. |
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Description | Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference. |
Final Product Type | illustra™ Single Cell GenomiPhi™ DNA Amplification Kit |
For Use With (Application) | Genomic DNA Amplification |
Includes | Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL |
Includes: Single Cell GenomiPhi Reaction buffer 1 x 275µL, Single Cell GenomiPhi Enzyme mix 1 x 25µL, Single Cell GenomiPhi Amplification mix 1 x 25µL, Single Cell GenomiPhi DTT Solution 1 x 100µL, Single Cell GenomiPhi Lysis buffer 1 x 100µL and Single Cell GenomiPhi Neutralisation buffer 1 x 100µL
Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
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Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
15301652
|
Cytiva
29108107 |
25 |
510.00 €
25 reactions |
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15311652
|
Cytiva
29108039 |
100 |
1833.00 €
100 reactions |
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Description
- For whole genome amplification of genomic DNA from as little as a single cell
- Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
- Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
- Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
- Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
- Reaction time: 2 to 4 hours
- Product average size: >10kb
For SNP arrays, Next Gen Sequencing, aCGH
Recommended StorageStore the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C
Specifications
Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C. | |
illustra™ Single Cell GenomiPhi™ DNA Amplification Kit | |
Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL | |
>10kb |
Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference. | |
Genomic DNA Amplification | |
illustra Single Cell GenomiPhi DNA Amplification Kit | |
2 to 4 hours |
Safety and Handling
- Danger
Hazard Category
- Skin corrosion/irritation Category 1
- Skin corrosion/irritation Category 1 B
Hazard Statement
- H314-Causes severe skin burns and eye damage.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : potassium hydroxide
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