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Invitrogen™ IgE Mouse ELISA Kit

Sandwich ELISA Kit

658.00 € - 2635.00 €

Specifications

Assay Sensitivity 0.14 ng/mL
Description IgE ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Other Proteins Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of IgE in Samples
Detectable Analytes Mouse IgE
Conjugate Biotin
Detection Method Colorimetric
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15405293
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Invitrogen™
EMIGHE
96 Tests
658.00 €
1 set
In Stock
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17105749
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Invitrogen™
EMIGHEX5
5 x 96 Tests
2635.00 €
Each
Estimated Shipment: 12-12-2024
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Description

Description

The Mouse Immunoglobulin E (Ms IgE) ELISA quantitates Ms IgE in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IgE. Principle of the method The Mouse IgE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.
Specifications

Specifications

0.14 ng/mL
Mouse IgE
Colorimetric
Other Proteins
This ELISA pair antibody detects mouse IgE. Other species not determined.
Mouse
Colorimetric Microplate Reader
<12%
HRP
Immunology
Mouse
4 hr. 45 min.
IgE ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Other Proteins Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of IgE in Samples
Biotin
ELISA Kit
Plasma, Serum, Supernatant
TMB
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Mouse
<10%
RUO
2°C to 8°C
1 hr. 20 min.
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