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Invitrogen™ Human TNF alpha ELISA Kits
Measure levels of human tumor necrosis factor alpha (TNFa) in human serum, plasma and cell culture supernatant samples using 96-well plates.
Brand: Invitrogen™ EH3TNFA2
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Description
Assay TNFα (human tumor necrosis factor alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Invitrgen™ Human TNF alpha ELISA Kits.
These human TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant human TNFα (human tumor necrosis factor alpha)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 15.6 to 1000pg/mL
Sensitivity: < 2pg/mL
Precision: Dependent upon sample type and level (see instructions)
Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3.5 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, TNFβ, Eotaxin, RANTES, GROα, GROβ, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, GCSF, GMCSF, MIP-1α, MIP-1β, TGFβ, IFNγ, IFNα; mouse TNFα; rat TNFα; and bovine TNFα. TNFα Receptor types 1 and 2 tested at 40mg/mL did not interfere in this assay. Recombinant pig TNFα cross-reacted at 100%
Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).
Specifications
| 15.6 to 1000pg/mL | |
| Human TNF alpha ELISA Kit | |
| Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum | |
| 2 x 96 microplate wells | |
| Absorbance at 450nm minus absorbance at 550nm | |
| 2 Plate Kit | |
| Natural and recombinant human TNFa (human tumor necrosis factor alpha) |
| <2pg/mL | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| No significant cross-reaction with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, TNFß, Eotaxin, RANTES, GROa, GROß, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, GCSF, GMCSF, MIP-1a, MIP | |
| Lyophilized Recombinant Human TNF alpha Standard, 4 vials, TMB Substrate Solution, 2 x 13mL, Streptavidin-HRP Reagent, 2 x 14mL, Sample Diluent, 2 x 13mL, Biotinylated Antibody Reagent, 2 x 13mL, Anti-Human TNF alpha Precoated 96-well Strip Plate, 2 ea, Adhesive Plate Covers, 12 ea, 30X Wash Buffer, 2 x 50mL, Stop Solution, 2 x 13mL | |
| Dependent upon sample type and level (see instructions) | |
| 50 μL | |
| 3.5 hr. |