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Invitrogen™ Caspase 8/FLICE Human ELISA Kit

For the quantitative detection of human Caspase-8.

Specifications

Accession Number Q14790
Assay Range 0.16-10.0 ng/mL
Assay Sensitivity 0.10 ng/mL
Conjugate HRP
Product Type ELISA Kit
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-8
anti-human Caspase-8 polyclonal (rabbit) Detection Antibody
Anti-rabbit-IgG-HRP
Human Caspase-8 Standard lyophilized, 20.00ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Lysis Buffer 10x
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

Description

The Human Caspase 8/FLICE ELISA quantitates Hu Caspase 8 in human serum, cell lysate or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Caspase 8. Principle of the method The Human Caspase 8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
Specifications

Specifications

Q14790
0.10 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
ALPS2B,CAP4,Casp-8,FLICE,MACH,MCH5
6.7%
HRP
Cell Lysate,50 μL; Serum, 50 μL; Supernatant, 50 μL
MACH, FADD-homologous ICE/CED-3-like protease
1 hr. 20 min.
0.16-10.0 ng/mL
HRP
Cell Lysates, Serum, Supernatant
ELISA
841
8.5%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Detection Antibody, anti-rabbit HRP conjugate, Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
Human
3 hr. 15 min.
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Product Certifications

For Research Use Only