missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. Cytiva HisTrap™ FF Columns: 16 x 25mm

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

197.00 € - 2343.00 €

Specifications

Diameter (English) Inner 0.6 in.
Diameter (Metric) Inner 16 mm
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate <20 mL/min.
Material Polypropylene
View More Specs

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10126414
View Documents
missing translation for 'viewProductCertificates'
Cytiva
17-5319-01 		5 x 1 mL
197.00 €
Pack of 5
In Stock
to see stock.
Edge
Add to basket
 
11595674
View Documents
missing translation for 'viewProductCertificates'
Cytiva
17-5319-02 		100 x 1 mL This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 		N/A
Description

Description

  • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
  • Column material: polypropylene
  • Bed dimensions: 16 × 25mm
  • Flow velocity: <20mL/min.
  • Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
  • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
  • Simple operation with syringe, perstaltic pump, or chromatographic system
  • Columns may be connected in series to increase capacity

Medium
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

Specifications

Specifications

0.6 in.
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Polypropylene
6% Cross-linked Agarose
5 bar (0.5 MPa, 70 psi)
16 mm
<20 mL/min.
3 to 12
4°C to 30°C, 20% Ethanol
Pre-packed Column
Videos
Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • EUH208-Contains nickel. May produce an allergic reaction.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
  • P405-Store locked up.

Supplemental information
  • MIXTURE LIST-Contains : ethanol, Nickel

SDS
Documents

Documents

Product Certifications
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.