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  6. Cytiva HisTrap™ excel Prepacked Columns
  7. 15187946

Cytiva HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

Brand:  Cytiva 29048586


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Regulatory: This product contains a substance listed in Annex XIV to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Product Code. 15187946

  • 70.00 € / 1mL
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Description

Description

The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.

  • Ready-to use prepacked with Ni Sepharose excel
  • Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
  • Made of biocompatible polypropylene and cannot be opened or refilled
  • Delivered with stopper on inlet and snap-off end on outlet
  • Operated either with peristaltic pump or with chromatography systems such as KTA systems
  • Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium
Media
  • Average particle size: 90μm
  • Matrix: Highly cross-linked agarose, 6%
  • pH Stability working range: 2 to 12
  • pH Stability cleaning range: 2 to 14
  • Flow velocity: 150 cm/h - 600 cm/h4
Column:
  • Column I.D.: 7 mm

HisTrap excel 1mL column

  • Bed Dimensions: 7 × 25mm
  • Flow rate: 1 to 4mL/min.
HisTrap excel 5mL column
  • Bed Dimensions: 16 × 25mm
  • Flow rate: 5 to 20mL/min.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

1 mL
7 mm
1 to 4 mL/min.
2 to 12
4°C to 30°C
1 mL
0.2 in.
HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Polypropylene
6% Highly Cross-linked Agarose
5 bar (0.5 MPa, 70 psi)
Pre-packed Column
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Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • LONG-TERM AQUATIC HAZARD Chronic 2
  • Flammable liquid Category 3
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : ethanol, nickel
  • UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.

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